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Real-time Live Imaging of T-cell Signaling Complex Formation
Published on: June 23, 2013
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Visualizing intracellular target antigens in live cells
Hiroshi Ueda1, Yancen Dai2, Farid Ghadessy3
1Graduate School of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta-cho, Yokohama, Kanagawa 226-8503, Japan.
Trends in Cell Biology
|February 9, 2023
Abstract:
In order to further visualize intracellular dynamics, precise imaging of endogenous proteins in live cells was performed using an antigen-binding fragment (Fab)-based Quenchbody (Q-body). The transfected Q-body probe showed an antigen-dependent fluorescence response, enabling the clear visualization and sorting of cells expressing p53, a tumor suppressor biomarker.
