Distribution of carbon tetrachloride-metabolite(s) to DNase I-sensitive and -resistant chromatin

The distribution of carbon tetrachloride (CCl4) metabolite(s) to DNase I-sensitive and -resistant regions of mouse hepatic chromatin was determined following incubation of whole chromatin with [14C]CCl4 in the presence of hepatic microsomes and an NADPH-regenerating system. At 2 h of incubation, [14C]CCl4 metabolite(s) bound essentially to the same extent to DNase I-sensitive and -resistant chromatin regions. Binding of [14C]CCl4 metabolite(s) to DNase I-resistant regions, however, increased significantly over the DNase I-sensitive regions when incubation was prolonged to 4 h. This result suggests that CCl4 does not bind preferentially to DNase I-sensitive regions of chromatin.