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The Current State of Nanopore Sequencing.
1Oxford Nanopore Technologies, Oxford, UK. jonathan.pugh@nanoporetech.com.
Methods in Molecular Biology (Clifton, N.J.)
|February 13, 2023
Summary
Nanopore sequencing offers a revolutionary method for analyzing DNA and RNA. This review covers its technology, scalability, and diverse applications in biological research as of June 2022.
Area of Science:
- Molecular Biology
- Genomics
- Biotechnology
Background:
- Nanopore sequencing, commercialized in 2015, represents a significant advancement in nucleic acid analysis.
- The technology enables the direct sequencing of native DNA and RNA molecules.
Approach:
- This review examines the fundamental principles and unique characteristics of nanopore sensing technology.
- It explores the functional mechanisms underlying nanopore sequencing.
Key Points:
- The platform's scalability is assessed, ranging from personal devices to high-throughput sequencing solutions.
- Current applications of nanopore sequencing across various scientific disciplines are highlighted.
Conclusions:
- Nanopore sequencing is a versatile and impactful technology with broad utility in biological research.
- Its ongoing development and adoption continue to drive innovation in genomics and molecular diagnostics.
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