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Region-resolved multi-omics of the mouse eye
Hang Xiang1, Bohan Zhang2, Yunzhi Wang1
1State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Institute of Biomedical Sciences, Human Phenome Institute, Zhongshan Hospital, Fudan University, Shanghai 200433, China.
Cell Reports
|February 15, 2023
Summary
Researchers created a detailed molecular map of the mouse eye, revealing region-specific proteins and lipids. This eye atlas provides a valuable resource for understanding vision and ocular diseases.
Area of Science:
- Ophthalmology
- Molecular Biology
- Proteomics
- Lipidomics
Background:
- The eye is a complex organ with specialized regions, and understanding its molecular organization is crucial for addressing vision impairment and blindness.
- Current knowledge of the hierarchical molecular network across different eye regions, including their functional divisions and interactions, remains incomplete.
Purpose of the Study:
- To create a comprehensive, region-resolved molecular atlas of the mouse eye, detailing its proteome and lipidome.
- To illuminate the division of labor and crosstalk among distinct anatomical regions of the eye at a molecular level.
Main Methods:
- Multiphoton microscopy-guided laser microdissection was employed to isolate specific eye regions.
- In-depth, label-free proteomics was performed to identify proteins.
- Integrative analysis combined spectral imaging, proteomics, and imaging mass spectrometry for lipidome and phosphoproteome profiling.
Main Results:
- Identification of 13,536 proteins across various mouse eye regions.
- Characterization of distinctive molecular features, including unique proteins and lipids specific to different anatomical regions.
- Generation of a comprehensive dataset integrating proteomic, lipidomic, and phosphoproteomic information.
Conclusions:
- The study presents the first region-resolved proteome and lipidome atlas of the mouse eye.
- This molecular atlas serves as a valuable resource for future research into eye function and the mechanisms underlying ocular diseases.
- An open proteome server was developed to integrate and provide access to all generated data for the scientific community.
