Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectrometers01:25

IR Spectrometers

1.2K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
1.2K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

269
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
269
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

486
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
486
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

552
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
552
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

3.4K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
3.4K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

1.4K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CODE MEDI: A Collaborative Hackathon for Health Informatics Education.

Studies in health technology and informatics·2026
Same author

Emerging strategies for controllable mechanical exfoliation of crystalline thin films and nanomembranes.

Nanoscale·2026
Same author

Liver transplantation is the major determinant of ≥10-year survival in patients with hepatocellular carcinoma.

Hepatology communications·2026
Same author

Nitride Micro-LEDs toward Biointegrated Platforms: Growth, Integration, and Emerging Applications.

ACS nano·2026
Same author

An intelligent and adaptive security framework for UAV swarms: a cross-layer approach integrating highly reliable EPUF, DRL-based key management, and distributed ledger technology.

Scientific reports·2026
Same author

Calcium Hydroxylapatite Injection Improves Senile Purpura via YAP-Mediated Mechanotransduction.

International journal of dermatology·2026
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Aug 10, 2025

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

4.0K

Spectrometer-based wavelength interrogation SPR imaging via Hadamard transform.

Soocheol Kim, Jin Hwa Ryu, Hoesung Yang

    Optics Letters
    |February 15, 2023
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel spectrometer-based wavelength interrogation surface plasmon resonance imaging (SPRi) method without mechanical scanning. This technique enables high-resolution refractive index mapping for advanced biosensing applications.

    More Related Videos

    High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
    10:40

    High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

    Published on: June 28, 2016

    7.6K
    Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
    10:42

    Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

    Published on: March 22, 2019

    6.3K

    Related Experiment Videos

    Last Updated: Aug 10, 2025

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
    09:46

    Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

    Published on: April 28, 2022

    4.0K
    High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
    10:40

    High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

    Published on: June 28, 2016

    7.6K
    Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
    10:42

    Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing

    Published on: March 22, 2019

    6.3K

    Area of Science:

    • Optics and Photonics
    • Biomedical Engineering
    • Materials Science

    Background:

    • Surface Plasmon Resonance Imaging (SPRi) is a label-free biosensing technique.
    • Traditional SPRi often requires mechanical scanning, limiting speed and complexity.
    • Developing faster, high-resolution SPRi methods is crucial for real-time biological analysis.

    Purpose of the Study:

    • To present a novel spectrometer-based SPRi method utilizing wavelength interrogation and a digital mirror device (DMD).
    • To eliminate the need for mechanical scanning in SPRi systems.
    • To demonstrate and validate the performance of the proposed SPRi technique.

    Main Methods:

    • Illumination of a gold-coated prism with polarized broadband light.
    • Spatial modulation of reflected light using a digital mirror device (DMD).
    • Spectrometer-based detection and reconstruction of reflectance spectral images via Hadamard transform (HT).
    • Refractive index (RI) mapping by analyzing resonance peak shifts in spectral images.

    Main Results:

    • Experimental evaluation of resolution, sensitivity, and dynamic detection range.
    • Achieved resolution of approximately 2.203 × 10-6 RI unit (RIU).
    • Measured sensitivity of approximately 3,407 nm/RIU and dynamic range of approximately 0.1403 RIU.
    • Simulations performed to validate experimental findings.

    Conclusions:

    • The proposed spectrometer-based SPRi method without mechanical scanning is feasible and effective.
    • The technique offers high resolution and sensitivity for refractive index mapping.
    • This advancement holds potential for label-free, real-time biosensing and imaging applications.