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Related Experiment Videos

Colicin E3 is an endonuclease.

S Ohno, K Imahori

    Journal of Biochemistry
    |December 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Colicin E3

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    Area of Science:

    • Molecular Biology
    • Bacteriology

    Background:

    • Colicin E3 is known to inactivate ribosomes by cleaving 16S rRNA.
    • The precise mechanism and specificity of this cleavage are not fully understood.

    Purpose of the Study:

    • To investigate the direct interaction of colicin E3's active components with isolated 16S rRNA.
    • To determine if colicin E3 directly degrades rRNA and to characterize this activity.

    Main Methods:

    • Polyacrylamide gel electrophoresis (PAGE) was used to analyze rRNA degradation.
    • Chromatography techniques, including Sephadex G-75, were employed for protein purification and activity assessment.
    • Inhibition assays using colicin E3 protein B were performed.

    Main Results:

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  • Isolated 16S rRNA was cleaved by colicin E3's active component (protein A) and fragment (T2A).
  • This rRNA degradation was random, unlike the specific cleavage on ribosomes.
  • Colicin E3 protein B inhibited rRNA degradation, confirming colicin E3's direct role.

    • Conclusions:

    • Colicin E3 directly degrades isolated 16S rRNA, although with lower activity and specificity compared to ribosome inactivation.
    • The active components of colicin E3 are responsible for this rRNA degradation.
    • These findings clarify the direct interaction of colicin E3 with its rRNA target.