Bioactive ECM-Loaded SIS Generated by the Optimized Decellularization Process Exhibits Skin Wound Healing Ability in...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Tissue Engineering

Background:

• Diabetic ulcers are a severe complication affecting 15-25% of diabetes patients.
• Conventional treatments like skin flaps have limited efficacy and risks.
• Skin tissue engineering offers a novel solution for diabetic ulcer treatment.

Purpose of the Study:

• To develop and evaluate a bioactive patch for diabetic ulcer treatment.
• To optimize a decellularization strategy for creating a cell-free scaffold.
• To assess the wound healing potential of the patch in a diabetic rat model.

Main Methods:

• Prepared a bioactive patch using porcine small intestinal submucosa (SIS) and extracellular matrix (ECM) from urine-derived stem cells (USCs).
• Optimized decellularization time and methods for scaffold preparation.
• Investigated in vitro wound healing mechanisms and assessed in vivo feasibility in a type I diabetic rat model.

Main Results:

• The developed patch demonstrated comparable effectiveness to USCs-loaded SIS in promoting skin wound healing.
• The optimized decellularization strategy retained bioactive ECM components.
• The patch showed feasibility for treating skin defects in diabetic rats.

Conclusions:

• The bioactive patch is a promising therapeutic strategy for diabetic ulcers.
• Optimized decellularization protocols can yield effective cell-loaded scaffolds.
• This approach offers a potential advancement in skin tissue engineering for wound regeneration.