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Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA
Mrityunjay Gupta1, Jingtian Wang2, Chely M Garfio2
1Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA. rspitale@uci.edu.
Abstract:
We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution via mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorporation across the cycloadduct site.
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