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Liquid Crystal Monomer: A Potential PPARγ Antagonist.

Haoduo Zhao1,2, Caixia Li2, Mihir Yogesh Naik3

  • 1School of Civil and Environmental Engineering, Nanyang Technological University, 639798 Singapore.

Environmental Science & Technology
|February 23, 2023
PubMed
Summary

Liquid crystal monomers (LCMs) can harm health by blocking PPARγ. This study reveals their toxic effects and mechanisms, aiding future chemical risk assessments.

Keywords:
AMPKPPARγ antagonistfatty acid β-oxidationglycolysisliquid crystal monomerreporter gene assaytrans-omicsvirtual screen

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Area of Science:

  • Environmental chemistry
  • Toxicology
  • Biochemistry

Background:

  • Liquid crystal monomers (LCMs) are prevalent in LCDs and e-waste.
  • Research on LCM environmental presence is growing, but toxic effects are understudied.

Purpose of the Study:

  • To comprehensively elucidate the toxicity and mechanisms of LCMs using integrated omics analysis.
  • To identify specific molecular targets and pathways affected by LCM exposure.

Main Methods:

  • Utilized in silico simulation, in vitro assays, and omics integration (metabolome, transcriptome).
  • Employed high-throughput virtual screening and reporter gene assays.
  • Analyzed LCM effects on HK2 cells.

Main Results:

  • Identified specific LCMs antagonizing peroxisome proliferator-activated receptor gamma (PPARγ).
  • Observed global metabolome and transcriptome dysregulation in HK2 cells.
  • Found significant dysregulation of fatty acid β-oxidation, potentially mediated by IL-17, TNF, and NF-kB pathways.

Conclusions:

  • LCMs act as potential PPARγ antagonists.
  • Discovered a trans-omics toxicological mode of action for LCMs.
  • Findings offer insights for future chemical risk assessments of LCMs.