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A New and Effective Method to Trace Tibetan Chicken by Amino Acid Profiling
Mengjie Qie1, Yalan Li1, Xiangyu Hu2
1Institute of Quality Standard & Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Foods (Basel, Switzerland)
|February 25, 2023
Summary
Geographical traceability of Tibetan chicken was determined using amino acid profiling. This method accurately identifies the origin of this nutritious, high-value food source, aiding food safety and preventing fraud.
Area of Science:
- Food Science
- Analytical Chemistry
- Animal Science
Background:
- Tibetan chicken is a nutritionally rich and medicinally valuable food source.
- Accurate geographical traceability is crucial for food safety and preventing fraudulent labeling of Tibetan chicken.
- Identifying the origin of Tibetan chicken helps authenticate its unique qualities.
Purpose of the Study:
- To establish a reliable method for determining the geographical origin of Tibetan chicken.
- To investigate the relationship between amino acid profiles and geographical location.
- To assess the potential of amino acid profiling for tracing plateau animal food products.
Main Methods:
- Analysis of amino acid profiles in Tibetan chicken samples from four distinct cities in Tibet, China.
- Application of chemometric techniques including orthogonal least squares discriminant analysis (OLS-DA), hierarchical cluster analysis (HCA), and linear discriminant analysis (LDA).
- Correlation analysis between amino acid concentrations and varying altitudes.
Main Results:
- High discrimination rates were achieved: 94.4% original and 93.3% cross-validation.
- Amino acid profiles effectively differentiated Tibetan chicken samples based on geographical origin.
- A positive correlation was observed between altitude and amino acid content, with a normal distribution pattern.
Conclusions:
- Amino acid profiling, combined with chemometrics, provides a highly accurate method for tracing the geographical origin of Tibetan chicken.
- This approach is effective for ensuring food safety and combating fraudulent practices.
- This study represents the first comprehensive application of amino acid profiling for tracing the origin of plateau animal food, demonstrating satisfactory accuracy.

