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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Untargeted Metabolomics Analysis Using UHPLC-Q-TOF/MS Reveals Metabolic Changes Associated with Hypertension in
Kexin Zhang1, Yanyan Liu2, Lingyun Liu1
1Beijing Municipal Key Laboratory of Child Development and Nutriomics, Capital Institute of Pediatrics, Beijing 100020, China.
Insights
Childhood hypertension mechanisms are unclear. This study found lipid metabolism disorders and altered metabolites in hypertensive children, particularly those who are overweight, suggesting their role in hypertension development.
Area of Science:
- Pediatrics
- Metabolomics
- Cardiovascular Health
Background:
- Hypertension in children is a growing concern with poorly understood underlying mechanisms.
- Identifying metabolic signatures could elucidate disease pathways and inform targeted interventions.
Purpose of the Study:
- To investigate plasma metabolomic profiles in children with different types of hypertension.
- To explore the potential mechanisms contributing to childhood hypertension, including simple and H-type variants.
Main Methods:
- Non-targeted metabolomics analysis of serum samples from control and hypertensive children (categorized by BMI and hypertension type).
- Identification and quantification of differential metabolites using advanced analytical techniques.
Main Results:
- 1235 differential metabolites identified between hypertensive children and controls, with 193 significantly altered metabolites, including various lipids.
- Significant lipid metabolism dysregulation observed, especially in overweight hypertensive children.
- Elevated levels of 3-dehydroepiandrosterone sulfate, oleic acid, linoleic acid, and NG,NG-dimethyl-L-arginine noted in specific hypertensive groups.
Conclusions:
- Childhood hypertension is associated with significant disruptions in lipid metabolism.
- Metabolomic alterations, particularly in lipid profiles and nitric oxide-related metabolites, play a crucial role in the pathogenesis of childhood hypertension.
Abstract:
The mechanism of hypertension in children remains elusive. The objective of this study was to analyze plasma metabolomics characteristics to explore the potential mechanism of hypertension in children. Serum samples from 29 control children, 38 children with normal body mass index and simple hypertension (NBp), 8 children overweight with simple hypertension (OBp), 37 children with normal body mass index and H-type hypertension (NH) and 19 children overweight with H-type hypertension (OH) were analyzed by non-targeted metabolomics. A total of 1235 differential metabolites were identified between children with hypertension and normal controls, of which 193 metabolites including various lipids were significantly expressed. Compared with the control group, 3-dehydroepiandrosterone sulfate, oleic acid and linoleic acid were up-regulated, and gamma-muricholic acid was down-regulated in the NBp group; 3-dehydroepiandrosterone sulfate, 4-acetamidobutanoate and 1-hexadecanoyl-2-octadecadienoyl-sn-glyero-3-phosphocholine were up-regulated in the OBp group, whereas adenosine and 1-myristoyl-sn-glyero-3-phosphocholine were down-regulated; in the NH group, 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine, phenol and 3-methoxytyramine were up-regulated, while pentadecanoic acid was down-regulated; in the OH group, NG,NG-dimethyl-L-arginine, 1-palmitoyl-sn-glycero-3-phosphocholine and monoethyl phthalate were up-regulated, while phloretin and glycine were down-regulated. The results showed that the children with hypertension had obvious disorders of lipid metabolism (especially in the overweight hypertension group), which led to the occurrence of hypertension. Additionally, the concentration of NO production-related NG, NG-dimethyl-L-arginine, was significantly increased, which may play an important role in H-type hypertension in children.
