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Quantify permeability using on-a-chip models in high-throughput applications.

Camilla Soragni1, Tessa Vergroesen2, Nynke Hettema2

  • 1MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands; Department of Cardiology, Maastricht University, P. Debyelaan 25, 6229 HX Maastricht, the Netherlands.

STAR Protocols
|March 2, 2023
PubMed
Summary

This study presents a modified exact equation for quantifying biological barrier permeability, accounting for time delays in assays. The new method improves accuracy for on-a-chip models, especially in leaky or cell-free conditions.

Keywords:
Biotechnology and bioengineeringCell-based AssaysHigh-throughput ScreeningMicroscopy

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Area of Science:

  • Biomedical Engineering
  • Pharmacokinetics
  • Drug Delivery

Background:

  • Traditional permeability quantification relies on sink conditions, which are often unmet in advanced in vitro models.
  • On-a-chip barrier models present unique challenges where standard assumptions fail, necessitating more robust quantification methods.

Purpose of the Study:

  • To develop an accurate and efficient protocol for quantifying biological barrier permeability using on-a-chip models.
  • To address the limitations of traditional methods by incorporating an exact solution modified for time delays.

Main Methods:

  • Utilized an exact permeability equation, adapting it to include a time offset.
  • Applied the modified equation to on-a-chip barrier models, evaluating performance in non-ideal conditions.

Main Results:

  • The modified exact equation accurately quantifies permeability even when sink conditions are not met.
  • Incorporating a time offset corrects for assay and data acquisition delays, enhancing precision.

Conclusions:

  • The presented protocol offers a more reliable method for permeability assessment in complex biological barrier models.
  • This advancement is crucial for accurate drug permeability studies and the development of predictive in vitro models.