Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

21.6K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.6K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
RNA-seq03:21

RNA-seq

10.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.2K
pre-mRNA Processing02:01

pre-mRNA Processing

53.1K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
53.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sequence-directed R-loop formation coupled with DNA methylation reprogramming during polyploidization of Brachypodium.

The Plant journal : for cell and molecular biology·2026
Same author

A cross-sectional study on the associations between serum metal exposure and thyroid hormones in chinese pregnant women.

Scientific reports·2026
Same author

Efficacy and safety of first-line osimertinib in Chinese patients with EGFR-mutated advanced non-small cell lung cancer: a prospective, multicenter, non-interventional study (FLOURISH).

Cancer biology & medicine·2026
Same author

Emerging roles of non-m<sup>6</sup>A mRNA modifications in plants.

Nature plants·2026
Same author

Women in plant science around the world.

Trends in plant science·2026
Same author

Effects of gestational exposure to individual and mixed PFASs on maternal liver function: evidence from the Wuxi Birth Cohort, animal studies, and toxicogenomic analyses.

Environment international·2026
Same journal

Somatic mobility of transposons is explosive and shaped by distinct integration biases in Arabidopsis thaliana.

Genome biology·2026
Same journal

UK Biobank whole-genome sequencing reveals robust contributions of rare variants to complex-trait heritability.

Genome biology·2026
Same journal

A one-week automated genome-wide optical pooled screen using OttoSeq.

Genome biology·2026
Same journal

Integrated lipidomic and transcriptomic profiling of the host response in human malaria.

Genome biology·2026
Same journal

Centromeric satellite expansion drives genome evolution in the snowy owl.

Genome biology·2026
Same journal

Mapping the landscape of allele-specific expression in porcine genomes.

Genome biology·2026
See all related articles

Related Experiment Video

Updated: Aug 7, 2025

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

8.4K

Recent advances in the plant epitranscriptome.

Lisha Shen1,2, Jinqi Ma3,4, Ping Li3

  • 1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore. lisha@tll.org.sg.

Genome Biology
|March 7, 2023
PubMed
Summary
This summary is machine-generated.

The plant epitranscriptome, including N6-methyladenosine (m6A) and 5-methylcytosine (m5C) modifications, regulates gene expression. This review covers recent advances in understanding these RNA modifications and their potential for crop improvement.

More Related Videos

An Easy Method for Plant Polysome Profiling
11:09

An Easy Method for Plant Polysome Profiling

Published on: August 28, 2016

12.7K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.2K

Related Experiment Videos

Last Updated: Aug 7, 2025

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
06:34

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

8.4K
An Easy Method for Plant Polysome Profiling
11:09

An Easy Method for Plant Polysome Profiling

Published on: August 28, 2016

12.7K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.2K

Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • The epitranscriptome, comprising chemical RNA modifications, plays a crucial role in gene regulation.
  • Recent advancements in sequencing and characterization of RNA modification machinery (writers, erasers, readers) have propelled the field of epitranscriptomics.
  • Understanding these modifications is key to deciphering complex biological processes.

Purpose of the Study:

  • To review recent progress in characterizing the plant epitranscriptome.
  • To highlight the regulatory roles of RNA modifications in post-transcriptional gene regulation and plant physiology.
  • To discuss the potential and challenges of epitranscriptome editing for crop improvement.

Main Methods:

  • Literature review of recent studies on plant epitranscriptomics.
  • Focus on N6-methyladenosine (m6A) and 5-methylcytosine (m5C) modifications.
  • Analysis of sequencing strategies and characterization of epitranscriptomic regulators.

Main Results:

  • The plant epitranscriptome involves widespread RNA modifications regulating gene expression.
  • Key modifications like m6A and m5C are involved in diverse physiological processes.
  • Epitranscriptome editing holds promise for agricultural applications.

Conclusions:

  • The epitranscriptome is a critical layer of gene regulation in plants.
  • Further research into m6A and m5C dynamics is essential.
  • Epitranscriptome editing presents exciting opportunities for crop enhancement.