Magnetic three-phase single-drop microextraction for highly sensitive detection of aflatoxin B₁ in agricultural...

In this work, a highly sensitive method for aflatoxin B₁ (AFB₁) detection was developed based on a peroxidase-like spatial network structure. The specific antibody and antigen of AFB₁ were coated on a histidine-modified Fe₃O₄ nanozyme to form the capture/detection probes. Based on the competition/affinity effect, the spatial network structure was constructed by the probes, which could be rapidly (8 s) separated by a magnetic three-phase single-drop microextraction process. In this single-drop microreactor, the network structure was applied to catalyze a colorimetric 3,3',5,5'-tetramethylbenzidine oxidation reaction for AFB₁ detection. The signal was amplified significantly due to the strong peroxidase-like ability of the spatial network structure and the enrichment effect of the microextraction. Thus, a low detection limit (0.034 pg/mL) was achieved. The matrix effect of real sample can be eliminated by the extraction approach, and the practicability of this method was proved by agricultural product samples analysis.