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Updated: Aug 7, 2025
08:06
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
Published on: January 19, 2017
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Replication timing and transcriptional control: beyond cause and effect - part IV.
Athanasios E Vouzas1, David M Gilbert2
1Department of Biological Science, Florida State University, Tallahassee, FL 32306-4295, USA.
Current Opinion in Genetics & Development
|March 11, 2023
Summary
Mammalian DNA replication timing (RT) is regulated by epigenetic feedback loops and cis-acting elements. This program is crucial for maintaining chromatin structure and plays a significant role in development.
Area of Science:
- Genetics
- Epigenetics
- Molecular Biology
Background:
- Spatiotemporal organization of mammalian DNA replication timing (RT) has correlations with transcription and chromatin organization.
- Mechanisms regulating RT and its biological significance were previously unclear.
Purpose of the Study:
- To review recent evidence on mechanisms controlling mammalian DNA replication timing.
- To explore the biological significance of RT regulation during development.
Main Methods:
- Review of recent scientific literature.
- Analysis of epigenetic mechanisms and cis-acting elements involved in RT regulation.
Main Results:
- The RT program is influenced by and necessary for maintaining chromatin structure, forming an epigenetic positive feedback loop.
- Specific cis-acting elements regulate RT at domain and whole-chromosome levels.
- Multiple cell-type-specific and developmentally regulated mechanisms of RT control have been identified.
Conclusions:
- Replication timing is a key epigenetic mechanism influencing chromatin organization and gene expression.
- Understanding RT regulation is crucial for comprehending developmental processes and maintaining genome stability.
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