Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Subviral Agents01:29

Subviral Agents

59
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
59
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

66
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
66
Retroviruses02:33

Retroviruses

12.6K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.6K
Introduction to Virus01:28

Introduction to Virus

116
Viruses are unique biological entities that blur the boundary between living and non-living systems. Although they lack cellular structure and metabolic processes, they can exhibit characteristics of life when infecting a host. Their defining feature is a nucleic acid core, composed of either DNA or RNA, encapsulated within a protein coat called a capsid. This simple structure allows them to invade host cells and use their machinery for replication efficiently.Viral Structure and...
116
Retrovirus Life Cycles01:10

Retrovirus Life Cycles

46.4K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
46.4K
Viral Recombination00:57

Viral Recombination

23.6K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interfacial Carrier Engineering of NiO/MS<sub>2</sub> Nanosheets for Electro-Oxidation of 5-Hydroxymethylfurfural with an Ampere-Level Current Density.

Journal of the American Chemical Society·2026
Same author

Machine Learning and Experimental Verification Identify Anti-Influenza Natural Products.

International journal of molecular sciences·2026
Same author

Unlocking Nonlinear Optics and Photoluminescence with Simultaneous Enhancement of Bandgap and Birefringence in Hybrid Sb-Based Halides.

Inorganic chemistry·2026
Same author

Machine learning model for predicting the risk of AKI in early hemodynamically stable sepsis patients: a study based on the MIMIC IV database.

Frontiers in medicine·2026
Same author

Convergent evolution of the N156K mutation in A(H1N1)pdm09 hemagglutinin contributes to antigenic drift and cluster transition.

Emerging microbes & infections·2026
Same author

Cathelicidin antimicrobial peptides: current progress and future prospects in immunotherapy.

Developmental and comparative immunology·2026

Related Experiment Video

Updated: Aug 6, 2025

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.1K

Pseudotyped Viruses.

Youchun Wang1,2, Zehua Zhou3, Xi Wu3

  • 1Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. wangyc@nifdc.org.cn.

Advances in Experimental Medicine and Biology
|March 15, 2023
PubMed
Summary

Pseudotyped viruses, which mimic authentic viruses, offer significant advantages for research. This review covers their construction, applications in viral analysis, and factors influencing their development.

Keywords:
Envelope proteinInhibitorNeutralizing antibodySelf-assembled pseudotyped virusVectorVirus entry

More Related Videos

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses
08:10

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Published on: January 15, 2020

8.4K
Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses
05:49

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Published on: November 21, 2023

1.8K

Related Experiment Videos

Last Updated: Aug 6, 2025

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
08:40

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

59.1K
Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses
08:10

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Published on: January 15, 2020

8.4K
Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses
05:49

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Published on: November 21, 2023

1.8K

Area of Science:

  • Virology
  • Molecular Biology

Background:

  • Pseudotyped viruses are engineered viral particles that display foreign envelope glycoproteins on their surface.
  • They serve as safer and more versatile alternatives to authentic viruses in various research applications.

Purpose of the Study:

  • To provide a comprehensive overview of pseudotyped virus technology.
  • To discuss their advantages, applications, and construction methodologies.

Main Methods:

  • Review of existing literature on pseudotyped virus construction and applications.
  • Analysis of different strategies and vectors used for pseudotyping.
  • Identification of key factors influencing pseudotyped virus generation.

Main Results:

  • Pseudotyped viruses effectively mimic authentic viruses, enabling diverse research uses.
  • Established methods and vectors facilitate their construction.
  • Understanding influencing factors optimizes their utility.

Conclusions:

  • Pseudotyped viruses are invaluable tools in virology research.
  • Their adaptability supports advancements in viral diagnostics and therapeutics.