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Updated: Aug 6, 2025
Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice
Published on: February 22, 2019
Immunization of MERS-CoV-2-infected mice with a sublethal dose of MERS-CoV or VRP-MERS-S
Jian Zheng1, Rudra Channappanavar2, Stanley Perlman3
1Department of Microbiology and Immunology, School of Medicine, University of Louisville, Louisville, KY 40202, USA; Center for Predictive Medicine, School of Medicine, University of Louisville, Louisville, KY 40202, USA; Departments of Microbiology and Immunology, University of Iowa, Iowa City, IA 52242, USA.
Abstract:
Here, we detail the immunization of mice with a sublethal dose of MERS-CoV or two doses of replication-incompetent alphavirus replicon particles expressing MERS-CoV spike protein. We then describe steps to determine the outcome of immunization by challenging immunized mice with a lethal dose of MERS-CoV, as well as by detecting virus-specific neutralizing antibody and virus-specific T cell response via neutralization assay and flow cytometry, respectively. This protocol can be used to evaluate other CoV infections or vaccine-induced immune responses. For complete details on the use and execution of this protocol, please refer to Zheng et al. (2021).1.
