Boron nitride nanofiber/Zn-doped hydroxyapatite/polycaprolactone scaffolds for bone tissue engineering applications

In this study, Zn doped hydroxyapatite (Zn HA)/boron nitride nanofiber (BNNF)/poly-ε-caprolactone (PCL) composite aligned fibrous scaffolds are produced with rotary jet spinning (RJS) for bone tissue engineering applications. It is hypothesized that addition of Zn HA and BNNF will contribute to cell viability as well as mechanical and osteogenic properties of the PCL scaffolds. Zn HA was synthesized by mixing Ca and P sources followed by sonication and aging whereas BNNF was obtained by the reaction of melamine with boric acid followed by freeze-drying for annealing of fibers. It is found that incorporation of both Zn HA and BNNF in PCL fibers resulted in higher calcium phosphate (CaP) precipitation on the scaffolds. Also, in vitro cell culture studies showed that presence of both Zn HA and BNNF also had synergistic effect for enhanced proliferation and osteogenic activity of Saos-2 cells. Mechanical properties of PCL-Zn HA-BNNF were found similar to that of non-load bearing bones. Furthermore, the presence of Zn HA and BNNF had synergistic effects to cell attachment, proliferation and spreading without causing cytotoxic effect on cells. The highest ALP activity was obtained in the PCL-Zn HA- BNNF group at days 7 and 14 due to release of zinc, calcium, phosphate and boron. Considering its mechanical and bioactivity properties, PCL-Zn HA-BNNF composite scaffolds hold promise as non-load bearing bone substitutes.