Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Problems of uridine protection.

S Farkas1, W Pfleiderer

  • 1Fakultät für Chemie, Universität Konstanz, FRG.

Nucleic Acids Symposium Series
|January 1, 1987
PubMed
Summary
This summary is machine-generated.

Researchers explored synthesis routes for a key oligoribonucleotide intermediate. Direct sulfonylation of uridine derivative (4) yielded the best results, though isomers were also produced.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Recommendations on treatment of acute appendicitis : Recommendations of an expert group based on the current literature].

Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2020
Same author

[3 D laparoscopy versus 2 D laparoscopy : An up to date evaluation].

Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2018
Same author

[Surgical technique for laparoscopic kidney donation].

Zentralblatt fur Chirurgie·2014
Same author

[Diagnosis of and therapy for hepatocellular carcinoma].

Zeitschrift fur Gastroenterologie·2013
Same author

FOXP3+ regulatory T-cells in renal allografts: correlation with long-term graft function and acute rejection.

Clinical nephrology·2011
Same author

Characterization of microvascularization of liver tumor lesions with high resolution linear ultrasound and contrast enhanced ultrasound (CEUS) during surgery: First results.

Clinical hemorheology and microcirculation·2010
Same journal

Nucleotide specificity of DNA cleavage by esperamycin/calichemicin antitumor antibiotics.

Nucleic acids symposium series·2018
Same journal

A model reaction for the repair of nucleic acids. The repair of thymidine bromohydrins.

Nucleic acids symposium series·2018
Same journal

Effects of base damages on DNA replication.

Nucleic acids symposium series·2018
Same journal

Effect of serine residue on the effectiveness of cationic polypeptide-based gene delivery.

Nucleic acids symposium series·2003
Same journal

Smart probe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator.

Nucleic acids symposium series·2003
Same journal

The engineering, structure, and DNA binding properties of a novel His4-type zinc finger peptide.

Nucleic acids symposium series·2003
See all related articles

Area of Science:

  • Organic Chemistry
  • Nucleic Acid Chemistry

Background:

  • Oligoribonucleotide synthesis requires specific protected uridine intermediates.
  • Efficient synthesis of these intermediates is crucial for advancing nucleic acid research.

Purpose of the Study:

  • To investigate and optimize synthetic routes for 5'-O-dimethoxytrityl-O4-p-nitrophenylethyl-2'-O-p-nitrophenylethylsulfonyluridine (7).
  • To identify the most effective method for producing this valuable intermediate for oligoribonucleotide synthesis.

Main Methods:

  • Investigated various synthetic pathways for the target compound (7).
  • Focused on the direct sulfonylation of 5'-dimethoxytrityl-O4-p-nitrophenylethyluridine (4).
  • Analyzed the reaction products, including isomers and byproducts.

Related Experiment Videos

Main Results:

  • Direct sulfonylation of compound (4) proved to be the most effective synthetic route.
  • Compound (7) was obtained, but in nearly equal amounts alongside its 3'-NPES isomer (8) and the 2',3'-di-O-NPES derivative (6).

Conclusions:

  • The direct sulfonylation method offers a viable, albeit not perfectly selective, route to the desired intermediate (7).
  • Further optimization may be needed to improve selectivity and yield of the target compound in oligoribonucleotide synthesis.