Feasibility of Coacervate-Like Nanostructure for Instant Drug Nanoformulation

Area of Science:

• Nanomedicine
• Materials Science
• Drug Delivery Systems

Background:

• Limited clinical translation of nanomedicine due to manufacturing and stability challenges.
• Scalable, cost-effective, and stable nanoformulation strategies are critical for clinical success.
• Current methods often require complex large-scale production and long shelf-life considerations.

Purpose of the Study:

• To develop a rapid and scalable method for nanoformulation (NF) production.
• To create a stable coacervate-like nanosystem for drug delivery.
• To evaluate the efficacy of the instant nanoformulation in delivering doxorubicin (Dox) to multidrug-resistant (MDR) cancer cells.

Main Methods:

• Instant formulation of nanoformulations (NF) using a polyelectrolyte coacervate-like system.
• Utilized anionic pseudopeptide poly(l-lysine isophthalamide) derivatives, polyethylenimine, and doxorubicin (Dox).
• Employed a simple "mix-and-go" approach by adding precursor solutions in seconds.

Main Results:

• Successfully formulated nanoformulations instantly using the coacervate-like system.
• Demonstrated enhanced intracellular delivery of doxorubicin (Dox) to patient-derived multidrug-resistant (MDR) cells.
• Showcased improved drug delivery within 3D tumor spheroids.

Conclusions:

• The "mix-and-go" coacervate-like nanosystem provides a feasible strategy for instant drug formulation.
• This technique can overcome challenges associated with large-scale production and shelf-life of nanomaterials.
• The developed system shows potential for widespread application in nanomedicine for enhanced drug delivery.