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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

490
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
490
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

479
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
479

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Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
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Analysis of Milk Microstructure Using Raman Hyperspectral Imaging.

Anastasiia Surkova1, Andrey Bogomolov1

  • 1Department of Analytical and Physical Chemistry, Samara State Technical University, Molodogvardeyskaya Street 244, 443100 Samara, Russia.

Molecules (Basel, Switzerland)
|March 29, 2023
PubMed
Summary
This summary is machine-generated.

Confocal Raman microscopy maps the chemical microstructure of milk, revealing the spatial distribution of fat, protein, and lactose. This advanced technique offers detailed insights into milk composition without bulk analysis.

Keywords:
Raman spectroscopyconfocal Raman microscopyhyperspectral imagingmilk qualitymultivariate curve resolutionprincipal component analysisspectral clustering

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Milk's complex colloidal structure hinders traditional optical spectroscopic analysis.
  • Existing methods often provide only bulk chemical information and require sample destruction.

Purpose of the Study:

  • To apply scanning confocal Raman microscopy for investigating milk's chemical microstructure.
  • To develop a method for spatially resolving major chemical components in milk.

Main Methods:

  • Utilized scanning confocal Raman microscopy to acquire hyperspectral images of milk samples.
  • Employed chemometric data analysis, specifically multivariate curve resolution, for spectral deconvolution.
  • Generated graphical maps illustrating the spatial distribution of fat, protein, and lactose.

Main Results:

  • Successfully mapped the spatial distribution of fat, protein, and lactose in various milk samples.
  • Demonstrated the capability of confocal Raman microscopy to analyze milk's chemical heterogeneity.
  • Validated the effectiveness of multivariate curve resolution for extracting information from complex spectra.

Conclusions:

  • Scanning confocal Raman microscopy offers a powerful, non-destructive tool for detailed chemical analysis of milk.
  • The developed method enables visualization of major component distribution, aiding in understanding milk's microstructure.
  • This approach has potential applications in advanced analysis of milk and dairy products.