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Resonance Raman Spectroscopy Tissue Oxygenation Measurements in Neonates.
Tazuddin Azmi Mohammed1, Russell Ray Moores1, Karen D Hendricks-Muñoz1
1Division of Neonatal Medicine, Department of Pediatrics, Children's Hospital of Richmond at Virginia Commonwealth University, Richmond, Virginia, USA.
Neonatology
|March 30, 2023
Summary
Resonance Raman spectroscopy (RRS) noninvasively measures microvascular oxygen saturation (RRS-StO2), correlating well with central venous oxygen saturation (SCVO2). This method provides normative data for preterm infants and shows RRS-StO2 increases after blood transfusion.
Area of Science:
- Biomedical Engineering
- Neonatal Physiology
- Medical Diagnostics
Background:
- Current pulse oximetry has limitations in assessing microvascular oxygen content.
- Resonance Raman spectroscopy (RRS) offers a noninvasive method for microvascular oxygen measurement.
- Understanding microvascular oxygenation is crucial, especially in vulnerable populations like preterm infants.
Purpose of the Study:
- To correlate Resonance Raman spectroscopy microvascular oxygen saturation (RRS-StO2) with central venous oxygen saturation (SCVO2).
- To establish normative RRS-StO2 values in healthy preterm infants.
- To evaluate the impact of blood transfusion on RRS-StO2.
Main Methods:
- Thirty-three buccal and thenar RRS-StO2 measurements were taken to correlate with SCVO2.
- Normative RRS-StO2 values were determined from 31 measurements in 28 subjects.
- Eight subjects were monitored for changes in RRS-StO2 following blood transfusion.
Main Results:
- Good correlations were observed between RRS-StO2 (buccal: r=0.692, thenar: r=0.768) and SCVO2.
- The median RRS-StO2 in healthy subjects was 76% (IQR 68.7-80.8).
- A significant increase of 7.8 ± 4.6% in thenar RRS-StO2 was noted post-transfusion.
Conclusions:
- RRS is a safe, noninvasive tool for monitoring microvascular oxygenation.
- Thenar RRS-StO2 measurements are more practical than buccal measurements.
- Further research is needed to confirm RRS-StO2 findings across various gestational ages and clinical settings.
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