Structural Changes in Liposomal Vesicles in Association with Sodium Taurodeoxycholate.
Deepak Kumar1, Abhishek Suna1, Debes Ray2
1Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Raebareli, Lucknow, 226002, India.
AAPS Pharmscitech
|April 3, 2023
Summary
Soy lecithin liposomes modified with sodium taurodeoxycholate (STDC) exhibit reduced vesicle size and transform into cylindrical structures. These enhanced liposomes demonstrate remarkable stability under various stress conditions, indicating potential for improved drug delivery.
Area of Science:
- Physical Chemistry
- Materials Science
- Biotechnology
Background:
- Liposomes, particularly those from soy lecithin (SL), are widely explored for drug delivery.
- Incorporating additives like edge activators enhances liposome stability and elasticity.
Purpose of the Study:
- To investigate the impact of sodium taurodeoxycholate (STDC), a bile salt, on the microstructure of soy lecithin liposomes.
- To understand how STDC influences liposome size, morphology, and stability.
Main Methods:
- Liposomes were prepared using the thin film hydration method.
- Characterization involved dynamic light scattering (DLS), small-angle neutron scattering (SANS), electron microscopy, and rheological techniques.
- Nuclear magnetic resonance (NMR) was used to ascertain hydrophobic interactions.
Main Results:
- Incremental addition of STDC reduced liposome vesicle size, attributed to edge-activation.
- At higher concentrations, STDC induced morphological transitions from spherical to cylindrical structures.
- Bilayer thickness remained consistent, indicating no dissociation, and SL-STDC structures showed high stability against thermal stress, electrolytes, and dilution.
Conclusions:
- STDC acts as an effective edge activator, modifying SL liposome microstructure and size.
- The observed morphological transitions are driven by STDC's hydrophobic interactions with the SL bilayer.
- Soy lecithin-STDC mixed structures exhibit enhanced stability, suggesting their utility in demanding drug delivery applications.
