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Published on: February 28, 2015
Rapid detection of dermatophytes using the Seegene Novaplex™ dermatophyte assay
Aline van Herk1, Cindy Houwen1, Martijn den Reijer1
1Star-shl Medical Diagnostic Center, Rotterdam, Netherlands.
Objectives:
We assessed the performance of the Seegene Novaplex™ Dermatophyte Assay for diagnosis of dermatophytosis.
Methods:
Sixty-one clinical samples from skin, nail, hair and culture were selected based on RT-PCR according to Wisselink et al. Of these samples, 26 samples were negative and 35 samples were positive with 39 dermatophytes strains. Emerging fungal strains harbouring terbinafine resistance (i.e. T. indotineae and T. mentagrophytes) were included.
Results:
The specificities of the Novaplex™ Dermatophyte Assay ranged between 94.3% and 97.9%. The sensitivities for the detection of T. rubrum complex, T. mentagrophytes/T. interdigitale species complex and C. albicans were 94.1% (95% CI: 71.3-99.9), 78.6% (95% CI: 49.2-95.3) and 100% (95% CI: 69.2-100), respectively, with Cohen's kappa of at least 72.9%.
Conclusions:
The Seegene Novaplex™ Dermatophyte Assay can be used for reliable screening of dermatophytes, including emerging strains in a routine laboratory setting.
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