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Stability of Vancomycin and Ceftazidime With Prolonged Storage at -20°C
Ophthalmic Surgery, Lasers & Imaging Retina
|April 6, 2023
Summary
Frozen vancomycin and ceftazidime are stable for up to six months at -20°C. This stability supports the practice of preparing and freezing intravitreal antibiotic doses for bacterial endophthalmitis treatment.
Area of Science:
- Ophthalmology
- Pharmacology
- Microbiology
Background:
- Bacterial endophthalmitis requires prompt antibiotic treatment.
- Intravitreal vancomycin and ceftazidime are standard treatments.
- Retinal practices often freeze aliquoted antibiotic doses.
Purpose of the Study:
- To assess the stability of vancomycin and ceftazidime after frozen storage.
- To validate the practice of pre-preparing and freezing intravitreal antibiotics.
Main Methods:
- Vancomycin and ceftazidime solutions were prepared and stored at -20°C.
- Samples were tested for stability at 3 and 6 months using high-performance liquid chromatography (HPLC).
- Stability was determined by comparing peak heights to freshly prepared reference samples.
Main Results:
- Vancomycin remained stable, with concentrations consistently near 100% over 6 months.
- Ceftazidime also demonstrated stability, with values generally above 100% throughout the 6-month period.
- No significant degradation was observed for either antibiotic under the tested conditions.
Conclusions:
- Both vancomycin and ceftazidime are stable when stored frozen at -20°C for up to 6 months.
- This supports the clinical practice of preparing and freezing intravitreal antibiotic doses.
- Frozen storage is a viable method for maintaining the integrity of these essential antibiotics.
