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Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

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When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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Proton (¹H) NMR: Chemical Shift01:07

Proton (¹H) NMR: Chemical Shift

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Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei...
1.8K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

427
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
427
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.1K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.1K
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

254
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
254
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

254
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
254

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Updated: Aug 1, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
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Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

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Hyperpolarized NMR metabolomics.

Victor Ribay1, Clément Praud1, Marine P M Letertre1

  • 1Nantes Université, CNRS, CEISAM UMR 6230, F-44000 Nantes, France.

Current Opinion in Chemical Biology
|April 24, 2023
PubMed
Summary
This summary is machine-generated.

Hyperpolarized NMR dramatically boosts signal detection for metabolomics, enabling the study of low-concentration metabolites in biological samples. This review explores advanced techniques and challenges for its widespread application.

Keywords:
Dissolution dynamic nuclear polarizationHyperpolarizationMetabolomicsNMR spectroscopyPara-hydrogen

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Last Updated: Aug 1, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Background:

  • Conventional Nuclear Magnetic Resonance (NMR) metabolomics faces sensitivity limitations, hindering the detection of low-concentration metabolites.
  • Hyperpolarized Nuclear Magnetic Resonance (NMR) offers a significant signal enhancement to overcome these sensitivity issues.

Purpose of the Study:

  • To review the application of hyperpolarization techniques for molecular omics sciences.
  • To discuss recent advancements and challenges in hyperpolarized NMR for metabolomics.

Main Methods:

  • Dissolution-dynamic nuclear polarization and parahydrogen-based techniques for signal enhancement.
  • Integration of hyperpolarization with fast multi-dimensional NMR and quantitative workflows.

Main Results:

  • Hyperpolarization techniques provide tremendous signal enhancement for NMR metabolomics.
  • Recent developments enable faster acquisition and quantitative analysis.

Conclusions:

  • Hyperpolarized NMR is a powerful tool for sensitive metabolomics.
  • Addressing challenges in high-throughput, sensitivity, and resolution is crucial for broader adoption.