A review of advances in aptamer-based cell detection technology

Area of Science:

• Biotechnology
• Analytical Chemistry
• Molecular Biology

Background:

• Cell detection is fundamental in life science research.
• Current antibody-based methods face limitations in preparation and stability.
• Aptamers present a promising alternative molecular recognition element.

Purpose of the Study:

• To review aptamer-based cell detection methods.
• To discuss principles, advantages, and applications of these methods.
• To highlight future trends in aptamer-based cell analysis.

Main Methods:

• Aptamer-fluorescent labeling
• Aptamer-isothermal amplification assay
• Electrochemical aptamer sensor
• Aptamer-based lateral flow analysis
• Aptamer-colorimetric assay

Main Results:

• Aptamers overcome antibody limitations like complex preparation and denaturation.
• Various aptamer-based assays demonstrate potential for sensitive cell detection.
• Different methods offer suitability for diverse analytical needs.

Conclusions:

• Aptamers are versatile tools for cell detection, offering advantages over traditional antibodies.
• Continued development aims for more accurate, economical, and rapid aptamer-based assays.
• This review serves as a reference for advancing aptamer applications in analytical science.