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Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
Published on: July 14, 2017
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Cell cycle controls long-range calcium signaling in the regenerating epidermis
Jessica L Moore1, Dhananjay Bhaskar1, Feng Gao1
1Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.
The Journal of Cell Biology
|April 27, 2023
Summary
Adult stem cells coordinate skin regeneration through dynamic calcium (Ca2+) signaling. This intercellular communication, involving specific cell cycle stages and connexin43, creates a feedback loop essential for tissue repair.
Area of Science:
- Cell Biology
- Stem Cell Biology
- Dermatology
Background:
- Skin homeostasis relies on stem cell communication for balanced regeneration.
- Studying stem cell signaling dynamics in live mice presents significant challenges.
Purpose of the Study:
- To investigate intercellular calcium (Ca2+) signaling patterns in adult stem cells during skin regeneration.
- To elucidate the mechanisms coordinating tissue-wide stem cell communication.
Main Methods:
- Live imaging of the mouse basal stem cell layer.
- Application of machine learning tools to analyze Ca2+ signaling patterns.
- Investigation of the role of G2 cells and connexin43 in signal coordination.
Main Results:
- Basal stem cells exhibit dynamic, localized intercellular Ca2+ signaling.
- Ca2+ signals are coordinated across thousands of cells, an emergent property of the stem cell layer.
- G2 cells initiate Ca2+ signaling, and connexin43 mediates tissue-wide coordination.
- Ca2+ signaling drives cell cycle progression, establishing a communication feedback loop.
Conclusions:
- Intercellular Ca2+ signaling is crucial for coordinating stem cell behavior during epidermal regeneration.
- Specific cell cycle stages and cell-cell junctions (connexin43) are key regulators of this communication.
- A feedback loop exists where Ca2+ signaling influences stem cell proliferation and tissue repair.
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