Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Aug 1, 2025

Traction Force Microscopy to Study B Lymphocyte Activation
09:28

Traction Force Microscopy to Study B Lymphocyte Activation

Published on: July 23, 2020

6.3K

Quantifying Immune Cell Force Generation Using Traction Force Microscopy.

Marcel Issler1,2, Huw Colin-York1, Marco Fritzsche3,4

  • 1Kennedy Institute for Rheumatology, University of Oxford, Oxford, UK.

Methods in Molecular Biology (Clifton, N.J.)
|April 27, 2023
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resolving the mechanical paradox of myelination.

Communications biology·2026
Same author

Metabolic profiling of steatotic liver disease by fluorescence lifetime imaging microscopy.

Communications medicine·2026
Same author

Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.

EMBO reports·2025
Same author

Protocol for lattice light-sheet time-lapse imaging of early post-implantation mouse embryos.

STAR protocols·2025
Same author

Mechanical force matters in early T cell activation.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Quantifying biomolecular organisation in membranes with brightness-transit statistics.

Nature communications·2024
Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Quantifying immune cell mechanical force is crucial for understanding cell function. This guide details using traction force microscopy (TFM) to measure forces, particularly at the T-cell immunological synapse.

Area of Science:

  • Cellular mechanics
  • Immunology
  • Biophysics

Background:

  • Immune cells generate mechanical forces essential for their functions.
  • Accurate measurement of these forces is needed to understand immune cell behavior.
  • Traction Force Microscopy (TFM) is a key technique in this field.

Purpose of the Study:

  • To provide a comprehensive guide for quantifying immune cell force generation.
  • To detail the application of TFM in studying immune cell mechanics.
  • To focus on TFM's utility in analyzing the T-cell immunological synapse.

Main Methods:

  • Detailed protocol for Traction Force Microscopy (TFM).
  • Application of TFM to measure forces generated by immune cells.
  • Specific focus on T-cell immunological synapse force mapping.
Keywords:
BiophysicsCytoskeletonImmunological synapseMechanical forcesMechanobiologyT cell activationTraction force microscopy

More Related Videos

Pattern Generation for Micropattern Traction Microscopy
09:26

Pattern Generation for Micropattern Traction Microscopy

Published on: February 17, 2022

2.3K
Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions
06:37

Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions

Published on: June 16, 2018

5.6K

Related Experiment Videos

Last Updated: Aug 1, 2025

Traction Force Microscopy to Study B Lymphocyte Activation
09:28

Traction Force Microscopy to Study B Lymphocyte Activation

Published on: July 23, 2020

6.3K
Pattern Generation for Micropattern Traction Microscopy
09:26

Pattern Generation for Micropattern Traction Microscopy

Published on: February 17, 2022

2.3K
Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions
06:37

Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions

Published on: June 16, 2018

5.6K

Main Results:

  • Demonstration of TFM's capability to quantify spatio-temporal force distribution.
  • Insights into mechanical force generation at the immunological synapse.
  • Establishment of a quantitative framework for immune cell force analysis.

Conclusions:

  • TFM is a powerful tool for dissecting immune cell mechanical functions.
  • Quantitative force measurements enhance understanding of immune cell interactions.
  • This methodology is vital for advancing immunological research.