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A Novel Intelligent Indicator Film: Preparation, Characterization, and Application
Bing Han1, Peifeng Chen1, Jiaxuan Guo1
1Department of Chemistry, College of Arts and Sciences, Northeast Agricultural University, Harbin 150030, China.
Molecules (Basel, Switzerland)
|April 28, 2023
Summary
This study developed an intelligent indicator film using whey protein isolate nanofibers (WPNFs), anthocyanin (ACN), pullulan (PU), and glycerol (Gly). The film changes color with pH, effectively indicating salmon spoilage and enhancing food safety.
Area of Science:
- Food Science and Technology
- Materials Science
- Biotechnology
Background:
- Intelligent indicator films are emerging in food packaging for quality monitoring.
- Whey protein isolate nanofibers (WPNFs) offer a promising base for functional films.
- Colorimetric indicators are crucial for real-time food spoilage detection.
Purpose of the Study:
- To develop and characterize an intelligent indicator film for detecting food quality changes.
- To utilize anthocyanin (ACN) as a pH-sensitive color indicator within a whey protein isolate nanofiber (WPNF) matrix.
- To assess the film's suitability for monitoring salmon freshness through pH-dependent color changes.
Main Methods:
- Preparation of a novel edible film using WPNFs, pullulan (PU), anthocyanin (ACN), and glycerol (Gly).
- Incorporation of ACN as a color indicator and Gly as a plasticizer.
- Evaluation of the film's color change in response to varying pH levels.
- Assessment of the film's performance in detecting changes in salmon quality parameters (pH, hardness, chewiness, resilience).
Main Results:
- The WPNFs-PU-ACN/Gly film exhibited improved hydrophobicity and oxidation resistance.
- A distinct color shift from dark pink to grey was observed with increasing pH.
- The film's color change correlated directly with the pH changes in deteriorating salmon.
- The colorimetric response was consistent with changes in salmon's texture properties.
Conclusions:
- The developed WPNFs-PU-ACN/Gly edible film is effective for sensing pH changes, making it suitable for monitoring salmon spoilage.
- This intelligent indicator film demonstrates potential for enhancing food safety and quality assurance in food packaging.
- The integration of natural colorants like ACN into WPNF-based films offers a sustainable approach to smart food packaging.

