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Related Experiment Video

Updated: Aug 1, 2025

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

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Published on: August 10, 2015

Automation and Optimization of Rat Heart Decellularization Using a Vibrating Fluid Column.

Dumitru-Daniel Bonciog¹, Mihaela-Ruxandra Lascu¹, Liliana Mâțiu-Iovan¹

¹Measurements and Optical Electronics Department, Politehnica University Timisoara, 300006 Timisoara, Romania.

Sensors (Basel, Switzerland)

|April 28, 2023

Summary

This study validates software for optimizing heart decellularization using a vibrating fluid column. The system automates the process, ensuring complete cell removal and reducing processing time for rat hearts.

Keywords:

Langendorff device biomedical images cardiac biomatrix heart decellularization heart discoloration heart simulation software application software optimization tissue engineering vibrating fluid column

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Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

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Published on: November 26, 2018

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Last Updated: Aug 1, 2025

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

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Published on: August 10, 2015

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

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Published on: June 9, 2022

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Published on: November 26, 2018

Area of Science:

Biomedical Engineering
Tissue Engineering
Regenerative Medicine

Background:

Decellularization is crucial for tissue engineering and regenerative medicine.
Optimizing decellularization protocols is essential for efficient tissue scaffold preparation.
Automating the decellularization endpoint ensures consistency and reproducibility.

Purpose of the Study:

To validate a software application for optimizing heart decellularization.
To automate the determination of the decellularization endpoint in rat hearts.
To enhance the efficiency of the decellularization process using a vibrating fluid column.

Main Methods:

Development and validation of image analysis software for simulated heart discoloration.
Optimization of a pressure-controlled Langendorff apparatus with a vibrating fluid column.
Utilizing sodium dodecyl sulfate for decellularization of rat hearts.
Employing ultraviolet spectrophotometry to quantify dye, DNA, and protein concentrations.

Main Results:

The software successfully automated the detection of simulated heart discoloration, correlating with decellularization.
The vibrating fluid column significantly reduced decellularization time in rat hearts.
Quantification of DNA and protein levels confirmed effective decellularization.

Conclusions:

The validated software provides an automated method for monitoring and controlling heart decellularization.
The optimized apparatus with a vibrating fluid column enhances decellularization efficiency.
This approach offers a reproducible and time-saving method for preparing decellularized heart tissue.