Mueller matrix polarization imaging and quantitative parameters analysis method

Mueller matrix polarization imaging is a new biomedical optical imaging method that can generate both polarization and isotropic intensity images of structures of the biological tissue sample surface. In this paper, a Mueller polarization imaging system in the reflection mode is described for obtaining the Mueller matrix of the specimens. Diattenuation, phase retardation, and depolarization of the specimens are derived by using the conventional Mueller matrix polarization decomposition method and a newly proposed direct method. The results show that the direct method is more convenient and faster than the conventional decomposition method. The polarization parameter combination method is then presented in which any two of the diattenuation, phase retardation, and depolarization parameters are combined, and three new quantitative parameters are defined in order to reveal more detailed anisotropic structures. The images of in vitro samples are presented to demonstrate the capability of the parameters introduced.