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Updated: Jul 31, 2025

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
Published on: May 10, 2020
A prime editor efficiently repaired human induced pluripotent stem cells with AR gene mutation (c.2710G > A; p.
Ruiqi Sun1, Yiqiang Cui1, Zhaode Liu1
1State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China.
Abstract:
Prime Editor (PE) is a precise genome manipulation technology based on the CRISPR-Cas9 system, while its application in human induced pluripotent stem cells (iPSCs) remains limited. Here, we established a repaired hiPS cell line (SKLRMi001-A-1) from hiPSCs with androgen receptor (AR) mutation (c.2710G > A; p.V904M). The repaired iPSC line expressed pluripotency markers, retained normal karyotype, showed the capability of differentiating into three germ layers and was absence of mycoplasma infection. The repaired iPSC line will help to elucidate the mechanism of androgen insensitivity syndrome (AIS) and benefit treatment for AIS in the future.
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