Identification and functional characterization of insulin-like peptides in a pine beetle

Insulin - including insulin-like peptides (ILPs), relaxins and insulin-like growth factors (IGFs) - is an evolutionarily conserved hormone in all metazoans. It is involved in various physiological processes, such as metabolism, growth, reproduction, lifespan and stress resistance. However, there are no reports on the functional role of ILPs in the Chinese white pine beetle, Dendroctonus armandi. In this study, we have cloned and identified two ILP cDNAs in D. armandi. The expression levels of DaILP1 and DaILP2 were significantly changed in different developmental stages. Both ILPs were expressed mostly in the head and fat body. Moreover, starvation induces the reduction of ILP1 mRNA level in adults and larvae, while ILP2 only in larvae of D. armandi, respectively. Additionally, RNA-interference (RNAi) using double stranded RNA to knock down ILP1 and ILP2 reduced the mRNA levels of the target genes, and caused a significant reduction in body weight of D. armandi. Moreover, silencing ILP1 led to an increase of trehalose and glycogen and significantly enhanced starvation resistance in both adults and larvae. The results show that the ILP signaling pathway plays a significant role in growth and carbohydrate metabolism of D. armandi and may provide a potential molecular target for pest control.