Related Experiment Video
Updated: Jul 31, 2025
14:09
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
7.0K
Mesoscale standing wave imaging
Shannan Foylan1, Jana Katharina Schniete1, Lisa Sophie Kölln1
1Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.
Journal of Microscopy
|May 8, 2023
Summary
Standing wave (SW) microscopy was enhanced to image over 16,000 cells across a large field of view. This advanced interference imaging method enables mesoscale visualization of fixed and living cells, including those under flow conditions.
Area of Science:
- Biophysics
- Optical Microscopy
- Cell Biology
Background:
- Standing wave (SW) microscopy provides high-resolution 3D imaging of cellular structures.
- Conventional SW microscopy is limited by a small field of view due to high-magnification objectives.
Purpose of the Study:
- To upscale SW microscopy from microscale to mesoscale imaging.
- To expand the field of view for SW imaging while maintaining high resolution.
Main Methods:
- Utilized the Mesolens with its unique low-magnification, high-numerical aperture design.
- Applied single-wavelength excitation and the multi-wavelength TartanSW method.
- Demonstrated imaging of fixed and living cells, including cells under flow.
Main Results:
- Achieved SW imaging with a field of view of 4.4 mm × 3.0 mm.
- Enabled imaging of over 16,000 cells in a single dataset.
- Successfully applied SW imaging to study cells under flow conditions.
Conclusions:
- Mesolens enables mesoscale SW imaging, significantly increasing the field of view.
- This upscaled method is applicable to diverse cell imaging applications, including dynamic flow studies.
- Offers a powerful new tool for large-scale cellular analysis.
Related Concept Videos
01:28Standing Waves in a Cavity
969
A household microwave and lasers are examples of standing electromagnetic waves in a cavity. When two conducting metal plates are placed parallel at the nodal planes, it creates a cavity where standing waves are formed. The cavity between the two planes is analogous to a stretched string held at the points x = 0 and x = L. Here, the distance 'L' between the two planes must be an integer multiple of half of the wavelength. The wavelengths that satisfy this condition are given by:
969
01:04Modes of Standing Waves: II
885
The starting point for expressing the modes of standing waves is understanding the boundary conditions that the waves must follow. The boundary conditions are derived from the physical understanding of how the standing waves are sustained, that is, how the vibrating particles of the medium behave at the boundaries imposed on them.
For a tube open at one end and closed at the other filled with air, the modes are such that there is always an antinode at the open end and a node at the closed end....
For a tube open at one end and closed at the other filled with air, the modes are such that there is always an antinode at the open end and a node at the closed end....
885
01:15Standing Electromagnetic Waves
1.6K
Electromagnetic waves can be reflected; the surface of a conductor or a dielectric can act as a reflector. As electric and magnetic fields obey the superposition principle, so do electromagnetic waves. The superposition of an incident wave and a reflected electromagnetic wave produces a standing wave analogous to the standing waves created on a stretched string.
Suppose a sheet of a perfect conductor is placed in the yz-plane, and a linearly polarized electromagnetic wave traveling in the...
Suppose a sheet of a perfect conductor is placed in the yz-plane, and a linearly polarized electromagnetic wave traveling in the...
1.6K
01:03Modes of Standing Waves - I
3.0K
A close look at earthquakes provides evidence for the conditions appropriate for resonance, standing waves, and constructive and destructive interference. A building may vibrate for several seconds with a driving frequency matching the building's natural frequency of vibration; this produces a resonance that results in one building collapsing while the neighboring buildings do not. Often, buildings of a certain height are devastated, while other taller buildings remain intact. This...
3.0K
01:07Interference and Superposition of Waves
5.3K
When two waves of the same nature occur in the same region simultaneously, they result in interference. Interference of waves implies that the net effect of the waves is the sum of the individual waves' effects. However, it does not imply that the individual waves affect the propagation of other waves.
Interference occurs in mechanical waves, such as sound waves, waves on a string, and surface water waves. Mechanical waves correspond to the physical displacement of particles. Hence,...
Interference occurs in mechanical waves, such as sound waves, waves on a string, and surface water waves. Mechanical waves correspond to the physical displacement of particles. Hence,...
5.3K