Related Experiment Video

Updated: Sep 21, 2026

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells
10:10

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

Published on: October 26, 2009

Enhancement of collagen-induced aggregation of platelets in whole blood

R Setiabudy-Dharma, Y Funahara

    Thrombosis Research
    |June 1, 1986
    PubMed

    In order to elucidate the features of platelet aggregation in whole blood, studies were carried out on human blood. The platelet aggregation reaction was monitored by counting the residual free platelet number with an electronic particle counter (Coulter). Platelets in citrated whole blood were aggregated by a very small amount of collagen which did not aggregate platelets in citrated plasma. Such enhancement was not observed if ADP or epinephrine was used. By addition of isolated erythrocytes to platelet rich plasma, enhancement of the platelet response to collagen was obtained. The erythrocyte membrane stabilizer, Dilazep, abolished the enhancement effect of erythrocytes. Those results indicated that erythrocytes enhanced the platelet response to collagen. Although participation of ADP from the erythrocytes in the enhancement was suggested, the results of ADP determinations on suspensions of erythrocytes indicated that other factors of the erythrocytes might be involved in the enhancement.

    More Related Videos

    Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation09:13

    Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation

    Published on: April 6, 2017

    Microfluidics in Assessing Platelet Function06:47

    Microfluidics in Assessing Platelet Function

    Published on: November 8, 2024

    Related Concept Videos

    Intracellular Signaling Affects Focal Adhesions01:17

    Intracellular Signaling Affects Focal Adhesions

    Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
    Some...
    Formation of the Platelet Plug01:22

    Formation of the Platelet Plug

    The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
    As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
    Clot Retraction and Fibrinolysis01:16

    Clot Retraction and Fibrinolysis

    After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.