Effect of ultrasonication on emulsion formulation, encapsulation efficiency, and oxidative stability of spray dried...

Present study was conducted to develop a stable chia oil emulsion using an ultrasound emulsification technique. Whey protein concentrate, gum Arabic, and xanthan gum stabilized layer by layer chia oil emulsion was developed using an electrostatic deposition. Single-layer and multilayer emulsion of chia oil was developed and their stability is compared. Developed emulsions were characterized by viscosity, stability, surface charge, and droplet size. Layer-by-layer emulsion showed the highest stability (98%) among all the formulations developed. Formulated single-layer and double-layer emulsions were spray dried and the respective powders were characterized for bulk density, tapped density, Hausner ratio, Carr's index, moisture content, color values, encapsulation efficiency, peroxide value, XRD, and SEM. Multilayer emulsion-based powder showed better flowability properties. The encapsulation efficiency of multilayer microparticles was found to be 93% with the lowest peroxide value of 1.08 mEq O₂/kg fat. XRD diffractogram of the developed microparticles revealed amorphous nature. The developed ultrasound layer-by-layer emulsification technique is an efficient technique for developing chia oil-loaded microparticles.