Related Experiment Video
Updated: Jul 29, 2025

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
Published on: January 26, 2017
Site-Directed Mutagenesis Protocol to Determine the Role of Amino Acid Residues in Polycomb Group (PcG) Protein
Diaa Al-Raawi1, Aditi Kanhere2
1Science Faculty, Sana'a University, Sana'a, Yemen.
Abstract:
Site-directed mutagenesis (SDM) is a technique in molecular biology and protein engineering that is widely used to determine the significance of specific residues involved in post-translational modifications (PTMs), protein structure, function, and stability. Here, we describe a simple and cost-effective polymerase chain reaction (PCR)-based SDM method. This method can be used to introduce point mutation, short addition, or deletions in protein sequences. Using polycomb repressive complex-2 (PRC2)-associated protein JARID2 as an example, we demonstrate how SDM can be used to study structural and consequently functional changes in a protein.
More Related Videos
10:26Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
Published on: January 16, 2015
09:58An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Published on: November 29, 2016
Related Concept Videos
In-vitro Mutagenesis
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...