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Related Experiment Video

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Multiple virus sorting based on aptamer-modified microspheres in a TSAW device.

Xianglian Liu1, Xuan Chen1, Yangchao Dong2

  • 1Xi'an, 710049 China State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University.

Microsystems & Nanoengineering
|May 22, 2023
PubMed
Summary
This summary is machine-generated.

This study presents a novel method for differentiating flaviviruses like dengue virus (DENV) and tick-borne encephalitis virus (TBEV) using aptamer-modified microspheres and acoustic sorting. This technique enables sensitive and rapid viral diagnosis with minimal cross-reactivity.

Keywords:
BiosensorsMicrofluidics

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Area of Science:

  • Biotechnology
  • Virology
  • Nanotechnology

Background:

  • Flavivirus infections, including dengue virus (DENV) and tick-borne encephalitis virus (TBEV), present overlapping symptoms, complicating differential diagnosis.
  • Existing diagnostic methods often lack the required sensitivity, speed, and specificity, leading to unreliable results and delayed treatment.
  • Effective separation of distinct viral particles from complex biological samples is essential for improving diagnostic accuracy.

Purpose of the Study:

  • To develop a simplified, sensitive, and rapid assay for the early-stage differential diagnosis of dengue and tick-borne encephalitis.
  • To create a method for specifically capturing and sorting DENV and TBEV from biological samples, minimizing cross-reactivity.

Main Methods:

  • Development of aptamer-modified polystyrene (PS) microspheres of varying diameters for specific capture of DENV and TBEV.
  • Utilization of a traveling surface acoustic wave (TSAW) device for sorting microspheres based on particle size.
  • Characterization of captured viruses using laser scanning confocal microscopy (LSCM), field emission scanning electron microscopy (FE-SEM), and reverse transcription-polymerase chain reaction (RT‒PCR).

Main Results:

  • The aptamer-modified microspheres effectively captured target viruses.
  • The TSAW device successfully sorted microspheres by size, demonstrating an effective and damage-free acoustic sorting process.
  • Subsequent characterization confirmed the integrity of viruses post-sorting, validating the method for further analysis.

Conclusions:

  • The developed aptamer-microsphere-based acoustic sorting system provides a promising strategy for the differential diagnosis of flaviviruses.
  • This method offers a damage-free and effective approach for sample pretreatment, enhancing the sensitivity and reliability of viral disease diagnosis.
  • The technique holds potential for application in early-stage diagnosis of viral infections with overlapping clinical manifestations.