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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Jul 29, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

High-content CRISPR screening.

Christoph Bock^1,2, Paul Datlinger¹, Florence Chardon³

¹CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.

Nature Reviews. Methods Primers

|May 22, 2023

Summary

This summary is machine-generated.

CRISPR screens offer unbiased gene function discovery for various applications. These powerful tools identify genes conferring drug resistance or sensitivity, advancing biological research and drug development.

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Last Updated: Jul 29, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

Area of Science:

Genetics and Genomics
Molecular Biology
Biotechnology

Background:

CRISPR screens enable unbiased interrogation of gene function across diverse species and applications.
Pooled CRISPR screens introduce genetic perturbations into cell pools, which are then subjected to biological challenges.
Effects are evaluated by sequencing guide RNAs, yielding ranked gene lists for sensitivity or resistance.

Purpose of the Study:

To describe the fundamental and advanced concepts of CRISPR screening.
To highlight CRISPR screening as a flexible and reliable method for biological discovery.
To emphasize high-content methods for detailed biological insights within screens.

Main Methods:

Pooled CRISPR screens with genetically encoded perturbations.
Cell proliferation under biological challenges (e.g., drug treatment, infection).
Sequencing-based counting of guide RNAs to assess perturbation effects.
Adaptations for gene activation, silencing, and manipulation.
High-content read-outs like single-cell RNA sequencing and spatial imaging.
Bioinformatic analysis tools for reproducibility.

Main Results:

CRISPR screens generate ranked gene lists indicating sensitivity or resistance to challenges.
Adaptations allow for precise gene manipulation (activation, silencing).
High-content read-outs provide unprecedented cellular detail.
CRISPR screening has elucidated molecular mechanisms in numerous fields.

Conclusions:

CRISPR screening is a versatile and powerful technology for biological discovery.
Its applications span basic biology, medical genetics, cancer research, immunology, and infectious diseases.
High-content CRISPR screening methods offer deep biological insights crucial for research and drug development.