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Related Experiment Video

Updated: Jul 29, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Light-field tomographic fluorescence lifetime imaging microscopy.

Yayao Ma¹, Luzhe Huang^1,2,3, Chandani Sen⁴

¹Department of Bioengineering, University of California, Los Angeles, CA, USA.

Research Square

|May 22, 2023

Summary

This summary is machine-generated.

Light field tomographic FLIM (LIFT-FLIM) accelerates 3D imaging by reducing scanning steps. This novel computational technique enhances molecular-level biological visualization and data acquisition efficiency.

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Last Updated: Jul 29, 2025

Fluorescence Lifetime Macro Imager for Biomedical Applications

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Published on: April 7, 2023

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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Published on: February 9, 2012

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Published on: October 20, 2017

Area of Science:

Biophotonics
Molecular Imaging
Computational Microscopy

Background:

Fluorescence Lifetime Imaging Microscopy (FLIM) visualizes biological samples at the molecular level.
Conventional FLIM faces challenges with long acquisition times, especially for 3D imaging, due to extensive scanning.
High-resolution lifetime mapping is crucial for understanding molecular interactions and cellular environments.

Conclusions:

LIFT-FLIM offers a highly data-efficient method for volumetric fluorescence lifetime imaging.
The technique enhances speed and sensitivity, enabling advanced biological research.
LIFT-FLIM has potential applications in basic and translational biomedical research.