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Zn-MOF hydrogel: regulation of ROS-mediated inflammatory microenvironment for treatment of atopic dermatitis
Lirong Qiu1, Chengcheng Ouyang1, Wei Zhang1
1School of Pharmacy, Nanjing Tech University, 30Th South Puzhu Road, Nanjing, 211816, China.
Abstract:
Atopic dermatitis (AD) is a chronic and recurrent inflammation disease associated with immune dysfunction. The high level of reactive oxygen species (ROS) causes high oxidative stress and further results in the deterioration of AD. At the same time, the ROS produced by bacterial infection can further aggravate AD. Here, the prepared PVA-based hydrogel (Gel) has a high ROS scavenging ability, and the antibacterial agent Zn-MOF(ZIF-8) loaded into the hydrogel shows a lasting and effective antibacterial activity. Thus, a Zn-MOF hydrogel (Gel@ZIF-8) is prepared to regulate ROS-mediated inflammatory microenvironment. In vitro experiments show that Gel@ZIF-8 has good antibacterial effect and cell biocompatibility. In the AD-induced mouse model, Gel@ZIF-8 can significantly enhance the therapeutic effect, such as reduce the thickness of epidermis, the number of mast cells and IgE antibodies. The results indicate that the ROS-scavenging hydrogel could treat the AD by regulating the inflammatory microenvironment, providing a promising treatment for managing AD.
Insights
This study developed a novel hydrogel loaded with Zn-MOF (ZIF-8) to combat atopic dermatitis (AD). The hydrogel effectively scavenges reactive oxygen species (ROS) and eliminates bacteria, offering a promising new treatment for AD.
Area of Science:
- Biomaterials Science
- Dermatology
- Immunology
Background:
- Atopic dermatitis (AD) is a chronic inflammatory skin condition linked to immune dysfunction and oxidative stress.
- Elevated reactive oxygen species (ROS) worsen AD, and bacterial infections exacerbate this condition.
- Current treatments for AD often have limitations in managing the complex inflammatory microenvironment.
Purpose of the Study:
- To develop and evaluate a novel hydrogel composite, Gel@ZIF-8, for treating atopic dermatitis.
- To investigate the hydrogel's capacity for scavenging ROS and its antibacterial properties.
- To assess the therapeutic efficacy of Gel@ZIF-8 in an in vivo mouse model of AD.
Main Methods:
- Preparation of a PVA-based hydrogel (Gel) incorporating Zinc-based Metal-Organic Framework (Zn-MOF, ZIF-8).
- In vitro assessment of ROS scavenging ability, antibacterial activity, and cell biocompatibility.
- In vivo evaluation of Gel@ZIF-8 treatment in an atopic dermatitis-induced mouse model.
Main Results:
- Gel@ZIF-8 demonstrated significant ROS scavenging and sustained antibacterial activity against relevant pathogens.
- In vitro studies confirmed good cell biocompatibility of the hydrogel.
- In the AD mouse model, Gel@ZIF-8 significantly reduced epidermal thickness, mast cell infiltration, and IgE antibody levels.
Conclusions:
- The developed Zn-MOF hydrogel (Gel@ZIF-8) effectively regulates the ROS-mediated inflammatory microenvironment in atopic dermatitis.
- Gel@ZIF-8 shows significant therapeutic potential for managing AD by mitigating key pathological markers.
- This ROS-scavenging and antibacterial hydrogel offers a promising new avenue for AD treatment.
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