Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparative assessment of endogenous, integrated, and exogenous biomethanation strategies: performance, microbial community and metabolic pathways.

Bioresource technology·2026

Same author

Ultra-Wide-Field Noninvasive Imaging Through Scattering Media Via Physics-Guided Deep Learning.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Precision medicine in bladder cancer: Redefining treatment paradigms through ADCs, immunotherapy, and molecular subtyping.

Critical reviews in oncology/hematology·2026

Same author

Correction: Comprehensive insight on immune landscape in intracerebral hemorrhage patients with single‑cell RNA sequencing: from blood to hematoma.

Journal of neuroinflammation·2026

Same author

Peroxiredoxins in Stroke: Friends and Foes.

Cells·2026

Same author

Pediatric infection-triggered encephalopathy syndromes: a <i>multidimensional</i> biomarker analysis.

Frontiers in neurology·2026

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 29, 2025

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Efficient large-scale single-pixel imaging.

Daoyu Li, Zhijie Gao, Liheng Bian

|May 23, 2023

Summary

This summary is machine-generated.

This study introduces a novel sparse single-pixel imaging (SPI) technique and a deep distribution optimization (D2O) algorithm. This enables efficient 1K resolution imaging with fewer measurements, overcoming limitations in large-scale applications.

More Related Videos

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Related Experiment Videos

Last Updated: Jul 29, 2025

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Lensless Fluorescent Microscopy on a Chip

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

Area of Science:

Optics and Photonics
Computational Imaging
Signal Processing

Background:

Single-pixel imaging (SPI) speed is limited by resolution and modulation times.
Efficient large-scale SPI faces significant challenges hindering widespread adoption.

Purpose of the Study:

To develop a novel sparse SPI scheme for high-resolution imaging with reduced measurements.
To introduce an efficient reconstruction algorithm for large-scale SPI.

Main Methods:

Analysis of Fourier coefficient importance for natural images.
Sparse sampling strategy based on polynomial descending probability.
Development of a lightweight deep distribution optimization (D2O) algorithm for reconstruction.

Main Results:

Demonstrated imaging at above 1K resolution with significantly reduced measurements.
Achieved robust recovery of sharp scenes within 2 seconds using the D2O algorithm.
Experimental validation of superior accuracy and efficiency compared to conventional methods.

Conclusions:

The proposed sparse SPI scheme and D2O algorithm effectively address the limitations of conventional SPI.
This advancement enables efficient, large-scale, high-resolution single-pixel imaging.
The technique shows promise for broader applications of SPI.