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Long Noncoding RNA ACTA2-AS1 Inhibits Cell Growth and Facilitates Apoptosis in Gastric Cancer by Binding with

Zuchao Hu1, Zhen Chen2, Wei Jiang2

  • 1The Second Ward of Surgery, Sinophram Hanjiang Hospital, Shiyan, Hubei, China.

Biochemical Genetics
|May 24, 2023
PubMed

Insights

Long non-coding RNA ACTA2-AS1 is underexpressed in gastric cancer (GC). Upregulating ACTA2-AS1 inhibits GC cell growth and promotes apoptosis by regulating the miR-6720-5p/ESRRB axis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer (GC) is a significant global health threat.
  • Aberrant expression of long non-coding RNAs (lncRNAs) is implicated in GC development.
  • The specific role of lncRNA ACTA2-AS1 in GC remains to be fully elucidated.

Purpose of the Study:

  • To investigate the function and mechanism of lncRNA ACTA2-AS1 in gastric cancer.
  • To analyze the correlation between ACTA2-AS1 expression and GC patient prognosis.
  • To explore the regulatory network involving ACTA2-AS1, miR-6720-5p, and ESRRB in GC.

Main Methods:

  • Bioinformatic analysis of GC and normal tissue gene expression and prognosis data.
  • Western blotting and RT-qPCR to assess mRNA and protein levels.
  • Cellular assays including EdU, CCK-8, flow cytometry, and TUNEL staining.
  • Molecular assays such as nuclear-cytoplasmic fractionation, FISH, RNA pulldown, luciferase reporter assays, and RIP assay.

Main Results:

  • LncRNA ACTA2-AS1 was found to be underexpressed in GC tissues and cell lines.
  • Overexpression of ACTA2-AS1 suppressed GC cell proliferation and induced apoptosis.
  • ACTA2-AS1 directly targets miR-6720-5p, leading to increased ESRRB expression.
  • ESRRB knockdown reversed the anti-tumor effects of ACTA2-AS1 overexpression.

Conclusions:

  • LncRNA ACTA2-AS1 functions as a tumor suppressor in gastric cancer.
  • ACTA2-AS1 exerts its antioncogenic role by modulating the miR-6720-5p/ESRRB axis.
  • ACTA2-AS1 may serve as a potential therapeutic target for gastric cancer treatment.
Keywords:
ACTA2-AS1ESRRBGastric cancermiR-6720-5p

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