Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cyclin B3 dsRNA Orchestrate Meiotic Progression in Porcine Oocytes.

Journal of molecular cell biology·2026
Same author

Phosphatase PPP1CC Regulates the First Lineage Segregation by GAS5 in Mouse Preimplantation Embryos.

Cell proliferation·2025
Same author

Construction of a Genetic Prognostic Model in the Glioblastoma Tumor Microenvironment.

Genes·2025
Same author

Biopsy of Porcine Blastocysts for Sex Identification and Transcriptome Sequencing Based on Laser-Assisted Microdissection.

Reproduction in domestic animals = Zuchthygiene·2025
Same author

NAD<sup>+</sup> Promotes Superovulation of Huaxi Cattle Through Regulation of Cumulus Cell Proliferation and Oocyte Maturation.

International journal of molecular sciences·2025
Same author

Integration of Multiomics Data Reveals Selection Characteristics of <i>ITGB1</i> That Are Associated with Size Differentiation in Pigs.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Jun 22, 2026

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
10:01

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Published on: September 19, 2018

9.1K

Multiplexed Gene Engineering Based on dCas9 and gRNA-tRNA Array Encoded on Single Transcript.

Chaoqian Jiang1,2, Lishuang Geng1,2, Jinpeng Wang1

  • 1Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China.

International Journal of Molecular Sciences
|May 27, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a CRISPR-based platform for multiplexed genome engineering, enabling simultaneous regulation of multiple genes. The system facilitates transcriptional control and epigenetic modifications for complex biological research.

Keywords:
CRISPR/Cas9multiplexed genome engineeringtRNA-gRNA arraytriplex sequence

More Related Videos

A Customizable Protocol for String Assembly gRNA Cloning STAgR
10:00

A Customizable Protocol for String Assembly gRNA Cloning STAgR

Published on: December 26, 2018

9.8K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.5K

Related Experiment Videos

Last Updated: Jun 22, 2026

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
10:01

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing

Published on: September 19, 2018

9.1K
A Customizable Protocol for String Assembly gRNA Cloning STAgR
10:00

A Customizable Protocol for String Assembly gRNA Cloning STAgR

Published on: December 26, 2018

9.8K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.5K

Area of Science:

  • Molecular Biology
  • Genetics
  • Synthetic Biology

Background:

  • Multiplexed genome engineering is crucial for understanding gene interactions and genetic networks.
  • Targeting multiple genomic loci simultaneously allows for comprehensive analysis of complex phenotypes.

Purpose of the Study:

  • To develop a versatile CRISPR-based platform for simultaneous, multiplexed genome engineering.
  • To enable multiple functions (activation, repression, methylation, demethylation) at multiple genomic loci from a single transcript.

Main Methods:

  • Fused RNA hairpins (MS2, PP7, com, boxB) to guide RNA scaffolds and cognate RNA-binding proteins to effectors.
  • Constructed multiple guide RNAs in a tandemly arrayed transfer RNA-guide RNA architecture on a single transcript.
  • Integrated triplex sequences for simultaneous expression of proteins and RNAs.

Main Results:

  • Demonstrated simultaneous, independent regulation of multiple target genes using paired RNA hairpins and RNA-binding proteins.
  • Successfully applied the system for transcriptional activation, repression, DNA methylation, and DNA demethylation at endogenous targets.
  • Utilized up to 16 individual CRISPR guide RNAs delivered on a single transcript.

Conclusions:

  • The developed platform offers a powerful tool for investigating complex synthetic biology questions.
  • This system facilitates the engineering of complex phenotypes for medical applications.
  • Provides a novel approach for simultaneous, multiplexed genome engineering with diverse functional outputs.