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Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
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Dark-field light scattering microscope with focus stabilization.

Anna Peters1, Zhu Zhang1, Sanli Faez1

  • 1Nanophotonics, Debye Institute for Nanomaterials Science, Utrecht University, 3584CC Utrecht, The Netherlands.

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|May 30, 2023
PubMed
Summary
This summary is machine-generated.

We designed an adaptable inverted microscope for dark-field scattering microscopy. Active feedback stabilizes the imaging plane for precise nanoparticle analysis, enabling future multi-modal imaging.

Keywords:
Cross-polarization microscopyDark-field imagingFocus stabilizationInverted microscope

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Area of Science:

  • Optical microscopy
  • Nanoparticle characterization
  • Scattering techniques

Background:

  • Developing versatile microscopy setups is crucial for advanced material and biological studies.
  • Dark-field microscopy offers high contrast for small or weakly absorbing samples.
  • Precise control over imaging conditions is essential for quantitative measurements.

Purpose of the Study:

  • To present a detailed design for a single-objective inverted microscope.
  • To enable two distinct dark-field microscopy modes: total internal reflection scattering and cross-polarization backscattering.
  • To implement active feedback for imaging plane stabilization.

Main Methods:

  • The microscope design utilizes a single objective lens.
  • Two dark-field modes are achieved by interchangeable mode-steering elements compatible with the Thorlabs cage system.
  • Active feedback control stabilizes the imaging plane for differential microscopy.
  • Long-term scattering measurements on single nanoparticles were performed to validate stabilization.

Main Results:

  • A versatile inverted microscope design is detailed.
  • The system supports switching between total internal reflection scattering and cross-polarization backscattering modes.
  • Active feedback successfully stabilized the imaging plane, validated by nanoparticle scattering measurements.
  • The setup demonstrates potential for extension to simultaneous scattering, fluorescence, and confocal imaging.

Conclusions:

  • The presented inverted microscope design offers flexibility for dark-field scattering applications.
  • Active feedback stabilization enhances measurement stability for nanoparticle studies.
  • The modular design facilitates future integration of multiple imaging modalities.