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Updated: Jul 28, 2025

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
Published on: January 9, 2017
Dark-field light scattering microscope with focus stabilization
Anna Peters1, Zhu Zhang1, Sanli Faez1
1Nanophotonics, Debye Institute for Nanomaterials Science, Utrecht University, 3584CC Utrecht, The Netherlands.
Abstract:
We present detailed design and operation instructions for a single-objective inverted microscope. Our design is suitable for two dark-field modes of operation: 1- total internal reflection scattering, and 2- cross-polarization backscattering. The user can switch between the two modes by exchanging one mode-steering element, which is also adapted to the Thorlabs cage system. To establish a stable background speckle for differential microscopy the imaging plane is stabilized with active feedback. We validate the stabilization efficacy by performing long-term scattering measurement on single nanoparticles. This setup can be extended for simultaneous scattering, fluorescence, and confocal imaging modes.
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