Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

1.8K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.8K
Forced Transdifferentiation01:28

Forced Transdifferentiation

1.9K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
1.9K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.0K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.0K
Lineage Commitment01:21

Lineage Commitment

3.0K
Commitment is the  process whereby stem cells:
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enforced MYC expression directs a distinct transcriptional state during plasma cell differentiation.

Life science alliance·2025
Same author

Correction to: Inherited CD19 Deficiency Does Not Impair Plasma Cell Formation or Response to CXCL12.

Journal of clinical immunology·2025
Same author

The PRL2 phosphatase up-regulates miR-21 through activation of the JAK2/STAT3 pathway to down-regulate the PTEN tumor suppressor.

The Biochemical journal·2024
Same author

Effects of cladribine on intrathecal and peripheral B and plasma cells.

Clinical and experimental immunology·2024
Same author

Spontaneous EBV-Reactivation during B Cell Differentiation as a Model for Polymorphic EBV-Driven Lymphoproliferation.

Cancers·2023
Same author

B-cell capacity for expansion and differentiation into plasma cells are altered in osteoarthritis.

Osteoarthritis and cartilage·2023
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jul 28, 2025

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

12.3K

Metabolic Reprogramming During B-Cell Differentiation.

Sophie Stephenson1, Gina M Doody2

  • 1Division of Haematology and Immunology, Leeds Institute of Medical Research, University of Leeds, Leeds, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 31, 2023
PubMed
Summary
This summary is machine-generated.

This study describes methods for in vitro differentiation of human B cells, detailing metabolic shifts during their conversion into antibody-secreting plasma cells. These methods allow for evaluation of metabolic reprogramming across B cell activation and differentiation stages.

Keywords:
B cellDifferentiationPlasma cellPlasmablast

More Related Videos

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.2K
Recombinant Retroviral Production and Infection of B Cells
09:19

Recombinant Retroviral Production and Infection of B Cells

Published on: February 18, 2011

14.2K

Related Experiment Videos

Last Updated: Jul 28, 2025

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

12.3K
Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

6.2K
Recombinant Retroviral Production and Infection of B Cells
09:19

Recombinant Retroviral Production and Infection of B Cells

Published on: February 18, 2011

14.2K

Area of Science:

  • Immunology
  • Cell Biology
  • Metabolic Research

Background:

  • B cell differentiation is a complex process involving significant metabolic changes.
  • Understanding these metabolic shifts is crucial for studying B cell function and antibody production.

Purpose of the Study:

  • To describe methods for in vitro differentiation of human B cells.
  • To incorporate transitional stages of B cell differentiation in vitro.
  • To facilitate the evaluation of metabolic reprogramming during B cell differentiation.

Main Methods:

  • In vitro differentiation of human B cells.
  • Incorporation of transitional stages: resting, activated (DNA synthesis/mitosis), and terminally differentiated (quiescent/secretory).
  • Evaluation of metabolic requirements at each differentiation phase.

Main Results:

  • Established methods for stepwise in vitro human B cell differentiation.
  • Characterized metabolic reprogramming accompanying B cell activation and plasma cell formation.
  • Provided a model to study metabolic changes during B cell to plasma cell transition.

Conclusions:

  • The described in vitro methods enable comprehensive study of B cell metabolic reprogramming.
  • These methods are valuable for investigating the metabolic demands of antibody secretion.
  • Further research can utilize these models to explore B cell-related diseases and therapies.