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Published on: April 17, 2018
PROTEIN SYNTHESIS IN THE FAT BODY OF THE FIFTH LARVAL INSTAR PHILOSAMIA CYNTHIA LARVAE-RELATION TO TWO FEEDING
1Université Claude Bernard LYON-I Départment de Biologie Générale et Appliquée Laboratoire Associé au C. N. R. S. ne 243 43, Boulevard du 11 Novembre 1918 69622 VILLEURBANNE CEDEX - FRANCE.
Abstract:
In order to investigate the determinism of the metabolic variations associated with developmental change, protein synthesis was measured in vivo, in the fat body of Philosamia cynthia. During the fifth larval instar two periods, the necessary feeding period and the facultative feeding period, which constitute decisive developmental periods of the insect were xamined. eAfter incorporation of [3 H]-glycine protein synthesis was estimated by a calculation which takes account of the dilution of the radioactive amino acids in the free amino acid pool. During the necessary feeding period (from ecdysis to day 4) the protein synthesis increased steadily whereas during the facultative feeding period (from day 4 to onset of spinning) the protein synthetic level decreased. Some preliminary hypotheses are suggested for the mechanism responsible for protein synthesis variations during a larval instar.
