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Electrolyzed catholyte water efficiently extracts compounds from brewer's spent grain, with optimal conditions varying by compound type. Pressure enhances polysaccharide and nitrogenous compound extraction but reduces sugars and phenolics.

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Area of Science:

  • Food Science and Technology
  • Biochemistry
  • Green Chemistry

Background:

  • Brewer's spent grain (BSG) is a rich plant-based byproduct with potential for valuable compound extraction.
  • Electrolytically activated water (EAW), specifically catholyte (pH 9.3), is explored as a sustainable extraction medium.

Purpose of the Study:

  • To investigate the efficacy of catholyte for extracting diverse organic compounds from BSG.
  • To determine optimal extraction parameters (pressure, time, temperature) for different compound classes.

Main Methods:

  • Brewer's spent grain was processed via mashing, filtration, and washing.
  • Extraction experiments were conducted using catholyte under atmospheric and excess pressure (0.5-1 atm).
  • High-Performance Liquid Chromatography (HPLC) was used for quantitative analysis of extracted compounds.
  • Mathematical analysis and correlation methods were applied to interpret results and identify formation regularities.

Main Results:

  • Catholyte extraction at atmospheric pressure and 50°C for 120 min yielded superior results for β-glucan, sugars, nitrogenous, and phenolic compounds compared to water extraction.
  • Excess pressure conditions (0.5-1 atm) increased non-starch polysaccharide and nitrogenous compound accumulation but decreased sugars and phenolics with longer extraction times.
  • Ultrasonic treatment with catholyte effectively extracted β-glucan and nitrogenous fractions, but not sugars or phenolics.
  • Syringic and vanillic acids were identified as key influencers in furan compound formation under different pressure conditions.

Conclusions:

  • Catholyte facilitates efficient extraction of carbohydrate, nitrogenous, and monophenolic compounds, particularly under pressure.
  • Flavonoid extraction efficiency is improved by reducing extraction time under pressure conditions.
  • The study highlights catholyte as a promising green solvent for valorizing brewer's spent grain.