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DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

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Related Experiment Video

Updated: Jun 27, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Microfluidic Acoustic Method for High Yield Extraction of Cell-Free DNA in Low-Volume Plasma Samples.

Alvaro J Conde^1,2, Ieva Keraite^2,3, Nicholas R Leslie²

¹Micronit B.V., Enschede, Netherlands.

Methods in Molecular Biology (Clifton, N.J.)

|June 10, 2023

Summary

Researchers developed a microfluidic system for rapid, cost-effective cell-free DNA extraction from blood plasma. This technology enhances cancer detection and monitoring through liquid biopsies, improving upon existing methods.

Keywords:

Acoustofluidics Cell-free DNA Liquid biopsy Microfluidics Micromixer

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Last Updated: Jun 27, 2026

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

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Published on: November 23, 2015

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

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Published on: September 9, 2020

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

Published on: September 16, 2022

Area of Science:

Biomedical Engineering
Molecular Diagnostics
Genomics

Background:

Cell-free DNA (cfDNA) analysis is crucial for cancer diagnosis and treatment monitoring.
Current cfDNA detection methods can be invasive, expensive, or time-consuming.
Microfluidic technologies offer potential for rapid, decentralized, and cost-effective cfDNA analysis.

Purpose of the Study:

To present a novel, simple microfluidic system for efficient cfDNA extraction from low-volume plasma samples.
To demonstrate the system's versatility for static or continuous flow and integration into lab-on-chip devices.
To evaluate the cfDNA capture efficiency compared to existing methods.

Main Methods:

Development of a microfluidic system utilizing a bubble-based micromixer.
Fabrication of custom components using rapid prototyping and 3D-printing services.
Extraction of cfDNA from plasma samples (≤500 μL).

Main Results:

The microfluidic system successfully extracted cfDNA from small plasma volumes.
The system demonstrated high versatility, suitable for various flow configurations and integration.
A tenfold increase in cfDNA capture efficiency was observed compared to control methods.

Conclusions:

The presented microfluidic system provides a simple, efficient, and cost-effective solution for cfDNA extraction.
This technology has significant potential for improving liquid biopsy applications in cancer diagnostics and monitoring.
The bubble-based micromixer design offers a versatile platform for decentralized molecular diagnostics.